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- First dimension: Isoelectric focusing (IEF) on immobilised pH gradient (IPG) gels — proteins migrate to their pI where net charge = 0.
- Second dimension: SDS-PAGE — proteins are separated by molecular mass.
Therefore \(X = 1.3 - 0.1 = 1.20\).
This is a classic presentation of Neonatal Respiratory Distress Syndrome (RDS), also called Hyaline Membrane Disease:
- Prematurity (36 weeks — late preterm)
- Grunting, retractions, nasal flaring, tachypnea
- Ground glass appearance + air bronchograms on CXR = pathognomonic of RDS
Pathophysiology: Deficiency of surfactant (produced by Type II pneumocytes from ~24 weeks, mature by ~35 weeks). Surfactant reduces alveolar surface tension. Without it:
\[\text{Surface tension} \uparrow \Rightarrow \text{Alveolar collapse} \Rightarrow \text{V/Q mismatch} \Rightarrow \text{Hypoxia}\]
- Treatment: Exogenous surfactant (beractant, poractant alfa) via endotracheal tube + respiratory support (CPAP/mechanical ventilation)
- Prevention: Antenatal corticosteroids (betamethasone 12 mg IM × 2 doses, 24 hrs apart) if delivery expected before 34 weeks — accelerates fetal lung maturity
Compare the two quantities below.
Column A: $(0.82)^2 \cdot (0.82)^3$
Column B: $(0.82)^6$
Using the rule of exponents: $(0.82)^2 \cdot (0.82)^3 = (0.82)^{2+3} = (0.82)^5$.
Now compare $(0.82)^5$ vs $(0.82)^6$.
Since $0 < 0.82 < 1$, multiplying by $0.82$ makes the number smaller. Therefore $(0.82)^5 > (0.82)^6$.
Column A is greater.
A Cohort Study (also called a prospective study) is an observational analytical study where:
- A group of exposed individuals and a group of unexposed individuals are identified
- Both groups are followed forward in time to compare the incidence of disease
- It calculates Relative Risk (RR)
Comparison of study designs:
| Study Type | Direction | Measure |
|---|---|---|
| Cohort | Forward (prospective) | Relative Risk (RR) |
| Case-Control | Backward (retrospective) | Odds Ratio (OR) |
| Cross-sectional | Single point in time | Prevalence |
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